First record of the teleomorphic state of Pestalotiopsis associated with the leather fern (Rumohra adiantiformis) in Guatemala
DOI:
https://doi.org/10.35622/j.rca.2026.01.001Keywords:
asexual reproduction, morphology, sexual reproduction, symptomsAbstract
The leatherleaf fern (Rumohra adiantiformis) is among the ten best-selling ornamental plants in Guatemala; by 2022, export sales reached US$120 million. In addition to its ornamental importance, it also holds medicinal value, although its production is limited by phytosanitary issues. The objective of this research was to identify the causal agents of the disease known as "chicharrón" (scorching) in leatherleaf fern. Symptomatic plants with necrotic areas on the leaflets were collected. The samples were placed in a humid chamber and observed every 24 hours. Plant tissue sections and scrapings were performed. Additionally, symptomatic plant tissue was placed in PDA (Potato Dextrose Agar) culture media. The culture medium was kept in an incubator at 25 ± 2 °C. The symptoms presented by the plants were leaf necrosis, typically starting in the apical region; these symptoms are commonly known as "chicharrón." Initial diagnostic results demonstrated the presence of the phytopathogenic fungus Pestalotiopsis sp. The sexual phase of the fungus Pestalotiopsis (Ascomycota: Amphisphaeriales) was also found on the plant, marking the first record of this phase in R. adiantiformis. Asci with ascospores were observed; the asci exhibited a slightly fusiform shape, brown color, and two transverse septa. These results provide important information for diagnostics in both anamorphic and teleomorphic phases.
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